Journal: Cell

Article Title: Elevated Calprotectin and Abnormal Myeloid Cell Subsets Discriminate Severe from Mild COVID-19

doi: 10.1016/j.cell.2020.08.002

Figure Lengend Snippet:

Article Snippet: CD38 , Fluidigm , Pro# 3167001B.

Techniques: Recombinant, Reverse Transcription, SYBR Green Assay, Staining, Functional Assay, Software

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: CyTOF antibody panel

Article Snippet: Anti-Human CD38 (HIT2)- 144Nd , Fluidigm , Cat# 3144014B; RRID:AB_2687640.

Techniques:

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: Selected genes involved in Tfh cell biology

Article Snippet: Anti-Human CD38 (HIT2)- 144Nd , Fluidigm , Cat# 3144014B; RRID:AB_2687640.

Techniques: Activation Assay

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: Distinct CD4 + T cell subsets contribute to the generation of Tfh with heterogeneous functional profiles (A) Mean fluorescence intensity of the CXCR5 marker expressed by CXCR5 + PD-1 + cells derived from (1) naive, (2) MemPD-1 neg , (3) MemPD-1 neg and Tfh. (B) Frequency of IL-21- and/or IFNγ-positive cells among CXCR5 + PD-1 + cells at day 3. (C – E) Representative flow plots showing CXCR3, ICOS, and CD40L expression by CXCR5 + PD-1 + cells (left panel) and frequency of CXCR3-, ICOS-, and CD40L-positive cells among CXCR5 + PD-1 + cells at day 3 (right panel). (F) Ex vivo cells or their respective Tfh D3 counterparts obtained after 3 days of splenocyte culture were co-cultured with autologous B cells for 7 days. (G) Box plots represent the frequency of CD27 + CD38 + cells among CD19 + cells, the concentration of total immunoglobulins and the absolute number of live B cells after co-culture. (H) Quantification of IgG1, IgG4, and IgA in the co-culture supernatants. Each symbol (A–H) represents an individual donor. (A–H) A Wilcoxon matched pairs test was performed, ∗, p < 0.05; ∗∗, p < 0.005; ∗∗∗, p < 0.001.

Article Snippet: Anti-Human CD38 (HIT2)- 144Nd , Fluidigm , Cat# 3144014B; RRID:AB_2687640.

Techniques: Functional Assay, Fluorescence, Marker, Derivative Assay, Expressing, Ex Vivo, Cell Culture, Concentration Assay, Co-Culture Assay

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

Figure Lengend Snippet: HIV infection shapes Tfh cell differentiation and functions (A) Splenocytes were stimulated according to the previously described protocol in the presence of HIV lab strain (Yu2b). (B) Representative flow plots of p24 staining among splenocytes after 3 days of culture with HIV or not. (C) RNA sequencing was performed on Tfh D3 cells derived from distinct CD4 + T cell subsets with and without HIV. Multidimensional scaling was performed to visually cluster different CD4 + T cell populations based on their transcriptional profile (8,593 genes). (D) RNA sequencing was performed on Tfh derived from each CD4 + T cell subset in the presence of HIV-1 infection or not. Differentially expressed genes were analyzed between Tfh and their original counterpart. Venn diagram representing (un)shared downregulated and upregulated genes. Genes specifically involved in Tfh cell biology were analyzed (referred to in ). (E) Contribution of each CD4 T cell subset to total Tfh generated after 3 days of splenocyte culture (%). Data are plotted as the mean percentage contribution of each ex vivo CD4 + T cell subset: (1) naive CD4 + T cells, (2) (3) memPD-1 neg/pos , and (4) Tfh to total Tfh D3 cells after splenocyte culture. (F) Representative flow plot of p24 staining in Tfh derived from distinct CD4 + T cell subsets. (G) Frequency of CD127-, CD27-, CD38-, FAS-, and ki67-positive cells among Tfh that are infected (p24 pos ) or not (p24 neg ). Each symbol (A–E) represents an individual donor. (E and G) A paired Student’s t test was performed, ∗p < 0.05, ∗∗p < 0.01. (F and G) A Wilcoxon matched pairs test was performed; ∗, p < 0.05; ∗∗, p < 0.005; ∗∗∗, p < 0.001.

Article Snippet: Anti-Human CD38 (HIT2)- 144Nd , Fluidigm , Cat# 3144014B; RRID:AB_2687640.

Techniques: Infection, Cell Differentiation, Staining, RNA Sequencing, Derivative Assay, Generated, Ex Vivo

Journal: iScience

Article Title: Naive and memory CD4 + T cell subsets can contribute to the generation of human Tfh cells

doi: 10.1016/j.isci.2021.103566

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Article Snippet: Anti-Human CD38 (HIT2)- 144Nd , Fluidigm , Cat# 3144014B; RRID:AB_2687640.

Techniques: Cell Analysis, Purification, Virus, Recombinant, Blocking Assay, Antibody Labeling, Transfection, Software

Journal: Cell reports

Article Title: High-dimensional profiling clusters asthma severity by lymphoid and non-lymphoid status

doi: 10.1016/j.celrep.2021.108974

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-Human CD38 (HIT2)-172Yb , Fluidigm , Cat# 3172007B; RRID:AB_2756288.

Techniques: Purification, Antibody Labeling, Flow Cytometry, Software, Staining, Blocking Assay